FastQCFastQC Report
Sat 18 Mar 2023
SRR4895459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775864
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83610.4708130802809224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG57050.32125207786181825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC38870.21887937364572962No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT20500.11543676768040798No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC18190.10242901483446931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT312400.0138.340841
GGTATCA313900.0137.693928
GTGGTAT314750.0137.322086
TGGTATC316650.0136.543557
GTATCAA316600.0136.451469
CAGTGGT320600.0134.995914
AGCAGTG320200.0134.948382
AGTGGTA320800.0134.777185
GCAGTGG327800.0131.929463
TCAACGC313300.028.20252410-14
ATCAACG312800.028.19699110-14
AGAGTAC315300.028.00679215-19
CAGAGTA315100.028.00172615-19
TACATGG316100.027.8021120-24
GTACATG318150.027.6682220-24
GCAGAGT319000.027.65036415-19
AGTACAT319400.027.52387620-24
CAACGCA321900.027.52059610-14
GAGTACA315850.027.2953520-24
ACATGGG319650.027.25011620-24
Produced by FastQC (version 0.11.5)