FastQCFastQC Report
Sat 18 Mar 2023
SRR4895458_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895458_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001289
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA2002650.0143.778841
TAGCGAC2003050.0143.750122
AGCGACT595950.0143.4693
AGCGACG594250.0143.237813
GCGACAT182250.0143.168914
AGCGACA531700.0143.01353
GCGACGT200300.0142.90974
GCGACTA165550.0142.706134
GCGACTG188000.0142.46364
GCGACGA159500.0142.075294
GCGACTT193750.0142.022924
CGACATG57000.0141.481455
AGCGACC292000.0141.366473
CGACGTA57350.0141.24525
CGACGAT57300.0141.242895
CGACTAT58000.0140.53055
CGACTAG54600.0140.453725
GCGACAA153050.0140.401124
CGACATA51150.0140.363315
CGACTGA50900.0139.922035
Produced by FastQC (version 0.11.5)