FastQCFastQC Report
Sat 18 Mar 2023
SRR4895458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001289
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107820.5387527738372618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT38360.19167646451861775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA26910.13446333837841512No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC24510.1224710673970626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT24040.12012258099654773No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA21410.10698105071281559No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT20900.10443269312927818No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC20100.10043526946882735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT400850.0138.787351
GGTATCA401400.0138.65288
GTGGTAT402150.0138.447896
TGGTATC403150.0138.140187
GTATCAA406300.0137.122339
AGTGGTA406700.0136.845925
CAGTGGT408600.0136.262424
AGCAGTG408200.0136.200182
GCAGTGG416750.0133.423193
ATCAACG400450.028.28513710-14
TCAACGC401450.028.26128810-14
AGAGTAC402800.028.06694215-19
CAGAGTA404500.027.96321915-19
TACATGG404750.027.87523720-24
CAACGCA407900.027.8320410-14
GTACATG406700.027.75574320-24
GCAGAGT409750.027.6154715-19
CGCAGAG410300.027.58546815-19
AGTACAT409350.027.57606120-24
GAGTACA403300.027.5363920-24
Produced by FastQC (version 0.11.5)