FastQCFastQC Report
Sat 18 Mar 2023
SRR4895457_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895457_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367043
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC1368650.0143.731522
AGGCTAA1368900.0143.705281
GCTAACG415400.0143.627883
GCTAACT410550.0143.572023
GCTAACA357900.0143.342093
CTAACTA114150.0142.959524
CTAACGT136800.0142.642684
GCTAACC187950.0142.641683
CTAACTG129700.0142.462634
CTAACGG134100.0142.45634
CTAACGA112650.0142.116584
TAACTAG35100.0141.855075
CTAACAG116450.0141.248114
CTAACAT118050.0141.223194
TAACGTG42500.0140.519015
TAACGAT40350.0139.981955
CTAACTT131350.0139.960924
TAACGTA39900.0139.93775
CTAACCG49700.0139.56184
TAACGGT44350.0139.362355
Produced by FastQC (version 0.11.5)