FastQCFastQC Report
Sat 18 Mar 2023
SRR4895457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367043
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68030.4976434537904075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC22750.1664175889127116No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG21850.1598340359447362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA215250.0138.088558
AAGCAGT217600.0136.547961
GTGGTAT218250.0136.256386
GTATCAA218950.0135.886499
TGGTATC219200.0135.633017
AGTGGTA223100.0133.229775
AGCAGTG223300.0133.094652
CAGTGGT224000.0132.790864
GCAGTGG228400.0130.091233
CGTGGTA950.004900675730.3031065
TCAACGC215200.028.25954810-14
ATCAACG215700.028.14065710-14
CAACGCA216900.028.05796810-14
AGAGTAC216250.028.0237215-19
GTACATG217400.027.84787620-24
CAGAGTA218250.027.79989415-19
TACATGG218950.027.65073420-24
CGCAGAG219800.027.55146415-19
AGTACAT219700.027.50391620-24
GCAGAGT220800.027.47883615-19
Produced by FastQC (version 0.11.5)