FastQCFastQC Report
Sat 18 Mar 2023
SRR4895456_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895456_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749529
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCATG750400.0143.732941
GCCATGC750700.0143.67552
CCATGCG229250.0143.277823
CCATGCA187300.0143.175633
CCATGCT203750.0143.128623
CATGCGA58400.0142.550254
CATGCAG59550.0142.334764
CATGCGT74400.0142.164754
CATGCTG67700.0141.992464
CATGCTA54800.0141.936044
CATGCGG75400.0141.901554
CATGCAT62350.0141.597524
ATGCGTA21700.0141.584495
ATGCGAT21650.0141.579125
ATGCGAA14500.0140.928185
CATGCTT63700.0140.290974
CCATGCC135700.0140.246923
CATGCCG37200.0140.230534
CATGCAA50150.0140.175194
ATGCGAG18550.0139.63885
Produced by FastQC (version 0.11.5)