FastQCFastQC Report
Sat 18 Mar 2023
SRR4895456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749529
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36670.48924057641532215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11540.1539633556540174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9980.13315028504567533No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT9270.12367766957649404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC9090.12127616142937765No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC8910.11887465328226125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCGGGCGCGGTGGCTC8670.1156726424194394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG8360.11153671172162785No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC8180.10913520357451147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA7980.10646686118882659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT126600.0136.413511
GGTATCA126750.0136.045368
GTGGTAT126650.0136.039156
TGGTATC128300.0134.34577
GTATCAA128500.0134.304649
AGTGGTA128900.0133.608695
CAGTGGT129850.0132.742034
AGCAGTG130950.0131.662122
GCAGTGG131650.0130.962053
TCAACGC127350.027.77033610-14
AGAGTAC126800.027.76592315-19
ATCAACG127050.027.73394610-14
CAGAGTA127850.027.54914915-19
TACATGG127650.027.52979920-24
GTACATG127850.027.50925420-24
CAACGCA128900.027.44756910-14
AGTACAT128450.027.32471720-24
GAGTACA127150.027.09457820-24
GCAGAGT130750.027.00416815-19
CGCAGAG130450.026.9890315-19
Produced by FastQC (version 0.11.5)