FastQCFastQC Report
Sat 18 Mar 2023
SRR4895455_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895455_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863847
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCT866000.0143.525281
GCACCTC866000.0143.525282
CACCTCG257800.0142.858253
ACCTCGA67750.0142.297944
CACCTCA227700.0142.27943
CACCTCT259350.0142.004463
ACCTCAT76050.0141.998784
ACCTCGT82300.0141.705374
ACCTCAG68750.0141.693224
ACCTCGG86850.0141.405674
ACCTCTG84750.0140.834754
CTCGTGA6400.0140.518396
CTCAGGT7900.0140.248036
ACCTCTA73700.0139.888444
CCTCGAG22200.0139.029655
TCGGATA1400.0138.751887
CCTCGTA24300.0138.265475
CCTCGGA21800.0137.620365
CCTCGAT21600.0137.56235
CCTCAGT24200.0137.350345
Produced by FastQC (version 0.11.5)