FastQCFastQC Report
Sat 18 Mar 2023
SRR4895455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863847
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44760.5181473108085113No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG20740.24008881202342544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC20680.23939424458266334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA14620.16924293306569335No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG10750.12444333313653923No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC10740.1243275718964122No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC10480.12131777965310986No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA9120.10557425099583606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123600.0137.590018
GTGGTAT123950.0137.259556
AAGCAGT124400.0136.660681
TGGTATC124900.0136.157937
GTATCAA126000.0134.912129
AGTGGTA127500.0133.494265
AGCAGTG127550.0133.285682
CAGTGGT127950.0133.081014
GCAGTGG131150.0129.791763
TCAACGC123900.028.02061510-14
ATCAACG123500.028.01813110-14
TACATGG124100.027.81887420-24
AGAGTAC124300.027.79148315-19
CAGAGTA124450.027.76955215-19
GTACATG125000.027.68768120-24
AGTACAT125500.027.47412920-24
CAACGCA126800.027.41381810-14
GAGTACA123900.027.27118120-24
GCAGAGT126800.027.22084215-19
CGCAGAG127500.027.06010415-19
Produced by FastQC (version 0.11.5)