FastQCFastQC Report
Sat 18 Mar 2023
SRR4895454_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895454_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940437
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGATA26860.1384224275253461No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCTGCGTTGATA23130.11919995341255604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAC1942200.0143.778612
AGATGTA1944950.0143.57531
ATGTACG593150.0143.014273
ATGTACA478350.0142.738663
TGTACGG190500.0142.66554
TGTACGT197800.0142.274964
TGTACTA147000.0142.24774
TGTACGA155350.0142.059234
GTACTAG47500.0141.942665
TGTACCG80400.0141.585044
TGTACTG184300.0141.530364
GTACGGT58650.0141.212865
TGTACAG156100.0141.007924
GTACGTA53850.0140.571415
GTACGAT52750.0140.092515
GTACGTG64550.0140.01045
TGTACAT161100.0139.936754
ATGTACC297500.0139.751833
TGTACCT78750.0139.343344
GTACAGT51200.0139.274195
Produced by FastQC (version 0.11.5)