FastQCFastQC Report
Sat 18 Mar 2023
SRR4895454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940437
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG356021.8347413495001383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA106090.5467325143769161No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA45530.23463786765558478No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC36350.1873289367292007No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT28020.14440046237007437No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA27840.14347283627347862No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC27380.14110223624884496No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC24470.1261056143538801No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG23730.12229204040120858No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA23550.12136441430461281No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA21510.11085131854319413No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT21290.10971755331402153No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA20950.10796537068711842No Hit
GTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAG20490.10559477066248478No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT20280.10451254021645641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT439850.096.889391
GGTATCA454250.093.6866158
GTATCAA456550.093.23049
AGTGGTA464000.091.6869665
GTGGTAT465100.091.516546
TGGTATC466250.091.259947
CAGTGGT470450.090.521694
AGCAGTG471800.090.236542
GCAGTGG479350.088.7852253
TATAGCG200.00629406328.792427105-109
CATGGGG155950.022.7642325-29
AACGCGA5200.022.43088545-49
CACGGAT5300.022.29154290-94
CGACCTA3450.022.12185345-49
ATGGGGG59650.021.69019925-29
GAAACCG7000.021.6032385-89
ATGGGAG44750.021.24949325-29
AAACCGC7250.020.85829285-89
ATGGGGC37050.020.49368325-29
TCGGAAC5700.020.20271575-79
Produced by FastQC (version 0.11.5)