FastQCFastQC Report
Sat 18 Mar 2023
SRR4895453_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895453_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372398
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTC1374500.0143.6982
ACAGATT1376200.0143.52051
AGATTCG412400.0143.417083
AGATTCA353050.0143.209733
AGATTCC192800.0142.50483
GATTCGA109400.0142.081334
GATTCGT147950.0142.074834
AGATTCT425100.0141.92563
GATTCAG114500.0141.911974
GATTCGG127750.0141.500934
GATTCAT124100.0140.907824
GATTCCG50050.0140.041084
GATTCTG131800.0139.937384
ATTCGTA44150.0139.522315
GATTCAA100200.0138.752244
ATTCGAG33950.0138.624025
GATTCGC32700.0137.761284
ATTCGAT41700.0137.538045
ATTCGGA32900.0137.361285
ATTCGTG46500.0137.268625
Produced by FastQC (version 0.11.5)