FastQCFastQC Report
Sat 18 Mar 2023
SRR4895453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372398
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68130.49643033580637685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC32600.23754042194756914No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA30930.2253719402097642No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG24620.17939402418248934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA206800.0137.619138
GTGGTAT208300.0136.5596
TGGTATC208700.0136.469717
AAGCAGT208300.0136.445651
GTATCAA209900.0135.620949
CAGTGGT212450.0133.99314
AGTGGTA213050.0133.548175
AGCAGTG213850.0132.938172
GCAGTGG217550.0130.577943
GTATCGA300.001992319771.976829
TCAACGC206200.028.169410-14
ATCAACG206450.028.07253510-14
AGAGTAC208500.027.8172315-19
CAGAGTA210100.027.62594615-19
TACATGG209400.027.60565420-24
GTACATG209600.027.59305220-24
CAACGCA211850.027.4792810-14
AGTACAT210650.027.4281720-24
GAGTACA208150.027.25266620-24
GCAGAGT213400.027.19199615-19
Produced by FastQC (version 0.11.5)