FastQCFastQC Report
Sat 18 Mar 2023
SRR4895452_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895452_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486476
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5760.11840255223279257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC488000.0143.398671
CACGACC488700.0143.193272
ACGACCT140300.0143.137853
ACGACCG171850.0142.934773
CGACCGT54550.0142.668884
GACCGAT17350.0142.611885
CGACCTA41450.0142.293824
CGACCGA42950.0142.18094
CGACCAT36300.0141.874114
ACGACCA107850.0141.854813
GACCTAT14250.0141.836565
CGACCTG48150.0141.017324
GACCGGT20000.0140.618845
CGACCTT42600.0140.309864
GACCGTA15500.0140.143195
CGACCGG58900.0139.947784
ACGACCC71700.0139.943193
GACCTGA12600.0139.85965
GACCTAG13950.0139.73075
GACCGTT19600.0139.084855
Produced by FastQC (version 0.11.5)