FastQCFastQC Report
Sat 18 Mar 2023
SRR4895452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486476
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22580.4641544495514681No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7830.16095346944145242No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC6920.1422475106685633No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA5590.11490803246203306No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT5480.11264687261036517No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG5170.10627451302839194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC5170.10627451302839194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63150.0136.85358
GTGGTAT63050.0136.84236
AAGCAGT63150.0136.681811
TGGTATC63450.0136.093037
GTATCAA64000.0135.485679
AGTGGTA63950.0134.803945
AGCAGTG64850.0132.876772
CAGTGGT65300.0132.017014
GCAGTGG68350.0126.2831953
ATCAACG63000.028.14404110-14
TCAACGC63000.028.14404110-14
CACGGAT2050.028.12384290-94
AGAGTAC63250.027.87352415-19
TACATGG62950.027.85376520-24
CAGAGTA63450.027.78566215-19
GTACATG63400.027.74689120-24
AGTACAT63850.027.59642820-24
GCAGAGT64550.027.31216615-19
GAGTACA63050.027.28444920-24
CAACGCA65600.027.07245310-14
Produced by FastQC (version 0.11.5)