FastQCFastQC Report
Sat 18 Mar 2023
SRR4895451_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895451_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862405
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGC1867350.0143.4872
AATGTTG1873600.0143.008361
TGTTGCA448500.0142.649413
TGTTGCG582400.0142.040373
GTTGCTA144300.0141.226294
GTTGCGA143300.0141.107474
GTTGCAT149550.0140.982354
GTTGCAG147350.0140.841634
TGTTGCT556800.0140.302663
GTTGCGT197750.0139.175834
TTGCGAT51150.0138.94625
GTTGCGG202300.0138.890234
GTTGCAA126050.0138.446914
GTTGCTG185300.0138.122964
TTGCTAG48450.0137.781075
TGTTGCC313900.0137.360143
TTGCGTA55800.0137.293755
TTGCGAG46050.0137.151385
TGCGTAA16400.0136.411776
TGCGATA16100.0136.272836
Produced by FastQC (version 0.11.5)