FastQCFastQC Report
Sat 18 Mar 2023
SRR4895451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862405
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG72530.3894426829824877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67620.36307892214636456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC50240.26975872594843764No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA44210.23738123555295437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG20460.10985795248616707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC19520.10481071517741845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA410700.0137.977268
AAGCAGT413050.0137.508941
GTGGTAT412750.0137.431446
TGGTATC413150.0137.29847
GTATCAA414950.0136.668129
CAGTGGT420900.0135.043854
AGTGGTA419950.0135.040925
AGCAGTG422100.0134.509542
GCAGTGG432450.0131.256973
TCAACGC412300.028.1201610-14
ATCAACG411100.028.09722110-14
CGTGGTA1804.985288E-627.9838315
CAACGCA416050.027.88745710-14
AGAGTAC413350.027.88250415-19
CAGAGTA415300.027.77237915-19
TACATGG415600.027.71143520-24
GTACATG415650.027.69771420-24
AGTACAT416350.027.59236320-24
CGCAGAG418400.027.54940815-19
GCAGAGT420400.027.42176815-19
Produced by FastQC (version 0.11.5)