FastQCFastQC Report
Sat 18 Mar 2023
SRR4895450_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895450_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3436025
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTC3441150.0143.684712
AATCCGT3441600.0143.665921
TCCGTCA896200.0142.88853
TCCGTCG1034900.0142.885033
CCGTCGA267800.0142.153724
CCGTCTA287600.0142.048784
TCCGTCT1015400.0142.029393
CCGTCAG279300.0141.83924
CCGTCGT366750.0141.565494
CCGTCAT310200.0140.72244
CCGTCAA257100.0140.262014
CCGTCTG308350.0140.213324
TCCGTCC530900.0139.942753
CGTCTAG93450.0139.896425
CGTCATA86400.0139.819565
CCGTCGG325050.0139.805084
CGTCGTA107550.0139.752325
CGTCAGT96850.0139.516955
CGTCGAT99900.0139.146625
CGTCGGA84150.0139.026465
Produced by FastQC (version 0.11.5)