FastQCFastQC Report
Sat 18 Mar 2023
SRR4895450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3436025
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA164630.4791292263589468No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT132740.38631849302609844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT89140.259427681696146No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC85840.2498235606551175No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG80420.2340495194301555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC73660.21437562299459403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT61740.17968437365851528No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTTCGTTTGTTCAACGAT55320.16099999272415072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCTTTAATTTATTAATG48500.14115147590602511No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT45760.13317714510226206No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC45590.13268238735166363No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA41290.12016792660123252No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC36560.10640201977575832No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT34680.10093058112208148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT809900.0139.385711
GGTATCA815750.0138.220618
GTGGTAT817500.0137.995166
TGGTATC819200.0137.743937
GTATCAA821350.0137.278219
CAGTGGT828500.0136.22384
AGCAGTG828850.0136.164182
AGTGGTA833000.0135.436055
GCAGTGG838050.0134.643623
TCAACGC814550.028.3616410-14
ATCAACG815750.028.2493410-14
TACATGG817750.028.1111920-24
AGAGTAC819450.028.10144615-19
CAGAGTA821100.028.06425915-19
CAACGCA826200.027.9704310-14
GCAGAGT827650.027.83172415-19
GTACATG828950.027.76437620-24
CGCAGAG829600.027.75242215-19
AGTACAT832200.027.59366620-24
ACATGGG829400.027.4941420-24
Produced by FastQC (version 0.11.5)