FastQCFastQC Report
Sat 18 Mar 2023
SRR4895449_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895449_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965569
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACACG275600.0142.73823
AAGGACA973700.0142.708541
GACACGT97750.0142.661684
GACACAT93500.0142.373934
GACACTT98800.0142.092354
GACACTG89400.0141.90094
AGGACAC982400.0141.444732
GGACACT296650.0140.808293
GACACAG77800.0140.213554
GACACGG82800.0140.176224
GGACACA270000.0140.128723
GACACGA74450.0139.853784
ACACTGT33400.0139.173455
GACACTC23350.0138.982474
CACGATA8200.0137.770486
ACACGTA30500.0137.071335
ACACGTG30100.0136.980425
ACACATG29700.0136.40255
CACGTAT11250.0136.237756
GACACCT36500.0136.22464
Produced by FastQC (version 0.11.5)