FastQCFastQC Report
Sat 18 Mar 2023
SRR4895449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965569
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44640.46231807359184063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT19890.20599252875765484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC19510.20205702544302892No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT16280.1686052472687089No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCTTTAATTTATTAATG10880.1126796738503411No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC10160.10522293072789204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151950.0138.827748
AAGCAGT152000.0138.602751
GTGGTAT153450.0137.564476
TGGTATC153500.0137.42597
GTATCAA154000.0137.073179
CAGTGGT155350.0135.8824
AGTGGTA155550.0135.661035
AGCAGTG155950.0135.276812
GCAGTGG158450.0133.187873
TCAACGC152200.028.2968510-14
ATCAACG151950.028.24867410-14
AGAGTAC152100.028.19242315-19
TACATGG152750.028.0579720-24
CAGAGTA153100.028.02708415-19
GTACATG154850.027.66816320-24
GCAGAGT155850.027.52330615-19
AGTACAT155300.027.4860220-24
GAGTACA152650.027.44446420-24
CAACGCA157900.027.40299210-14
CGCAGAG158350.027.12513215-19
Produced by FastQC (version 0.11.5)