FastQCFastQC Report
Sat 18 Mar 2023
SRR4895448_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895448_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120167
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA1120950.0143.800611
CGAGTAA1121050.0143.787782
GAGTAAT390400.0143.16613
GAGTAAG356500.0142.894183
GAGTAAC113750.0142.511733
GAGTAAA271000.0140.770373
AGTAACG35150.0140.014044
AGTAATG134500.0139.784154
AGTAATA111700.0139.394264
AGTAAGA99200.0138.535954
AGTAATC42850.0138.530034
AGTAAGT111400.0138.284124
AGTAAGG129250.0137.835454
AGTAAGC28950.0137.441334
AGTAATT113650.0137.129154
AGTAAAT88150.0137.046894
GTAATAG40200.0136.922935
AGTAACC28900.0136.185324
AGTAACT27900.0135.135014
GTAACGT12250.0135.092915
Produced by FastQC (version 0.11.5)