FastQCFastQC Report
Sat 18 Mar 2023
SRR4895448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120167
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63240.5645586774114931No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG36260.32370173375934125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC25580.22835880721356724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT18620.16622521463317524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT14000.12498136438584605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC12190.1088230594188188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT271250.0138.748431
GTGGTAT272800.0138.109566
GGTATCA273300.0137.883228
TGGTATC274250.0137.458077
GTATCAA275300.0136.750819
AGCAGTG276550.0136.271592
CAGTGGT277050.0136.146794
AGTGGTA277450.0135.820825
GCAGTGG281400.0133.974093
TCAACGC272050.028.2641910-14
ATCAACG271850.028.23203510-14
CAACGCA274350.028.03248610-14
AGAGTAC274700.027.93463715-19
TACATGG274500.027.93400820-24
CAGAGTA275450.027.85857815-19
GTACATG275500.027.84306720-24
CGCAGAG276200.027.77250715-19
GCAGAGT276300.027.75203515-19
AGTACAT277200.027.63595620-24
AACGCAG279500.027.52111610-14
Produced by FastQC (version 0.11.5)