FastQCFastQC Report
Sat 18 Mar 2023
SRR4895447_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895447_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070580
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATC2074550.0143.623892
GAGATCG639850.0143.237923
GAGATCA524600.0143.215593
AAGAGAT2082100.0143.10311
GAGATCT672700.0142.77833
AGATCGA172150.0142.731084
AGATCGT231150.0142.438374
GAGATCC247450.0141.95323
AGATCTA185550.0141.846184
AGATCAG163350.0141.78714
AGATCAT183750.0141.630264
AGATCTG202850.0141.418464
AGATCAA150550.0141.177234
GATCGAG52600.0141.16565
GATCGAT62000.0140.535925
GATCGTA66800.0140.23925
GATCATG57150.0139.494935
AGATCCG66300.0139.451924
GATCGTG72750.0139.35195
AGATCGG191400.0139.277564
Produced by FastQC (version 0.11.5)