FastQCFastQC Report
Sat 18 Mar 2023
SRR4895447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070580
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107500.5191782012769369No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG58490.2824812371412841No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC42940.2073815066309923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG41260.19926783799708295No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGTGGTGGCTCA29570.14281022708613048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC24990.12069082092940142No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT24330.1175033082517942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT381400.0138.465771
GGTATCA381900.0138.095818
GTGGTAT383650.0137.50346
TGGTATC384950.0137.039057
GTATCAA385700.0136.809899
CAGTGGT391050.0135.122224
AGCAGTG391300.0134.925752
AGTGGTA393100.0134.27115
GCAGTGG406900.0129.823653
TCAACGC381550.028.3235410-14
ATCAACG381100.028.29277410-14
AGAGTAC382350.028.09273515-19
CAGAGTA382550.028.0818115-19
TACATGG384700.027.95339620-24
GTACATG387050.027.8208720-24
AGTACAT388600.027.64321320-24
GCAGAGT390650.027.52165215-19
CAACGCA393750.027.44595510-14
GAGTACA383700.027.38084420-24
CGCAGAG394300.027.2887915-19
Produced by FastQC (version 0.11.5)