FastQCFastQC Report
Sat 18 Mar 2023
SRR4895446_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895446_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41227
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC500.12127974385718097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGT5250.0143.991524
CCGGATA351.0913936E-11143.991527
ACCTAGA351.0913936E-11143.991526
CATGGTT151.2559674E-4143.99158
CTATGGA100.0072153783143.99158
ACCGGTG550.0143.99156
CCGGGAT306.657501E-10143.99157
CGTATTA100.0072153783143.99158
TCACCAC500.0143.99154
CGAGTTA100.0072153783143.99158
CGAGTGT202.1806427E-6143.99158
CCGATTA202.1806427E-6143.99157
CCTAGTA100.0072153783143.99157
CCGATCG100.0072153783143.99157
CCGATAG151.2559674E-4143.99157
ACCATAT202.1806427E-6143.99156
ACCATAG151.2559674E-4143.99156
CCAGGCA100.0072153783143.99157
CCAGGAT151.2559674E-4143.99157
ACCGCGA100.0072153783143.99156
Produced by FastQC (version 0.11.5)