FastQCFastQC Report
Sat 18 Mar 2023
SRR4895446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41227
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1330.3226041186601014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1200.29107138525723436No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.29107138525723436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT940.22800591845150023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC910.22072913382006937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCAGGCCGTTTGGCATCTC810.1964731850486332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT790.19162199529434595No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA740.17949402090862784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCTTTAATTTATTAATG730.1770684260314842No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAATTATTTGCCT570.13825890799718632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG550.13340771824289907No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTAGCTTTTACTTTTTA470.11400295922575011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACTA100.007215197143.992725
AAGCAGT10550.0137.502171
AGTGGTA10600.0135.842185
GGTATCA10650.0135.204428
GTATCAA10650.0135.204429
GTGGTAT10700.0134.572636
TGGTATC10750.0133.946727
AGCAGTG10850.0133.700262
GCAGTGG11050.0130.62723
CAGTGGT11050.0130.310154
GGGATCT255.353844E-428.79854625-29
GATACCC200.00627346228.798544105-109
TCAACGC10600.028.11933110-14
AGAGTAC10550.027.97962815-19
ATCAACG10650.027.85211210-14
GCAGAGT10600.027.71180515-19
CAGAGTA10650.027.58170315-19
AACGCAG10900.027.4775110-14
CAACGCA10900.027.34540710-14
ACATGGG10650.027.31129520-24
Produced by FastQC (version 0.11.5)