FastQCFastQC Report
Sat 18 Mar 2023
SRR4895445_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895445_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448287
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA1449900.0143.747822
TTCACGC1451000.0143.638841
ACGCATG146750.0143.372214
CACGCAT473600.0143.364623
ACGCATA136600.0143.01614
ACGCAAT123900.0142.691994
CACGCAG443200.0142.564043
ACGCATT148950.0142.36574
ACGCAGT142850.0142.350074
CGCATTA42150.0142.204455
CACGCAA383700.0142.148793
CGCATGA40000.0142.11275
CGCATAG43600.0141.601075
CGCATAT45550.0141.226075
CGCAATA37450.0141.029515
CGCAGTA42300.0141.019745
ACGCAGA121550.0141.010854
ACGCATC43450.0140.765084
ACGCAAG129900.0140.698784
CGCAGAT41350.0140.431265
Produced by FastQC (version 0.11.5)