FastQCFastQC Report
Sat 18 Mar 2023
SRR4895445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448287
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65800.45432983932052146No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG31470.2172911860701643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC30380.20976505347351732No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT29020.20037464949971934No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT14880.10274206700743706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA298650.0137.96578
AAGCAGT300050.0137.498611
GTGGTAT300150.0137.348146
GTATCAA300400.0137.090099
TGGTATC301150.0137.011577
CAGTGGT304700.0135.368034
AGTGGTA305500.0134.919315
AGCAGTG306350.0134.576972
GCAGTGG315650.0130.657563
TCAACGC298000.028.2136410-14
ATCAACG297950.028.12658310-14
AGAGTAC299650.028.04022815-19
TACATGG300350.027.92276620-24
CAGAGTA301450.027.86324715-19
CAACGCA302950.027.78590610-14
GTACATG303450.027.67072320-24
CGCAGAG305000.027.505915-19
AGTACAT304800.027.47731220-24
GCAGAGT306050.027.42093815-19
GAGTACA300350.027.38108820-24
Produced by FastQC (version 0.11.5)