FastQCFastQC Report
Sat 18 Mar 2023
SRR4895444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381469
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA142350.5977403023092049No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC69620.2923405679435676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG66040.2773078297471015No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT46970.1972312047731883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT27130.11392128136037043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA400000.0138.288338
TGGTATC403600.0137.072667
GTGGTAT404000.0136.883516
GTATCAA405550.0136.360359
AAGCAGT407100.0136.077941
CAGTGGT408850.0135.418174
AGTGGTA413000.0133.865725
AGCAGTG413950.0133.808762
GCAGTGG422350.0131.096333
TCAACGC400150.028.22643310-14
ATCAACG399500.028.20029810-14
AGAGTAC402200.027.97590615-19
CAGAGTA405000.027.80381615-19
TACATGG403700.027.7849720-24
GTACATG404850.027.75227420-24
AGTACAT408450.027.50414520-24
CAACGCA411100.027.481610-14
GAGTACA402200.027.39819120-24
GCAGAGT411900.027.33805715-19
CGCAGAG414850.027.11936615-19
Produced by FastQC (version 0.11.5)