FastQCFastQC Report
Sat 18 Mar 2023
SRR4895443_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895443_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835879
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCA1840200.0143.57542
TGGCTTC1843100.0143.34951
GCTTCAG574200.0143.276473
GCTTCAT603900.0143.117253
GCTTCAA480950.0142.703723
CTTCATG194100.0141.987824
GCTTCAC189750.0141.640243
CTTCAGT188300.0141.164154
CTTCAGG184800.0140.527984
CTTCAGA164200.0140.453574
CTTCAAT164500.0139.759984
CTTCACG53250.0139.185994
CTTCAAG155350.0138.959384
CTTCATA178900.0138.566024
CTTCATT185200.0136.844154
TTCATAG58950.0136.591645
CTTCAGC49500.0134.46664
TTCAGTG62300.0134.213425
TTCAGAG54100.0133.67395
TTCATGA52500.0133.635915
Produced by FastQC (version 0.11.5)