FastQCFastQC Report
Sat 18 Mar 2023
SRR4895443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835879
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104220.5676844715800986No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC50600.275617292860804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG45980.2504522356865567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT24490.1333965909518002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT444700.0139.252061
GGTATCA449800.0137.69428
GTGGTAT450300.0137.557286
TGGTATC451200.0137.31487
GTATCAA452550.0136.9379
AGCAGTG454850.0136.017992
CAGTGGT456000.0135.900964
AGTGGTA456550.0135.626885
GCAGTGG461900.0133.988713
TCAACGC448600.028.3248810-14
ATCAACG448900.028.22578210-14
AGAGTAC452050.028.05457115-19
TACATGG452650.027.94214220-24
CAGAGTA453600.027.93966515-19
CAACGCA455100.027.93930410-14
GTACATG453250.027.93056520-24
AGTACAT453950.027.83040620-24
CGCAGAG457550.027.67644115-19
GCAGAGT458200.027.64974615-19
AACGCAG463950.027.4125610-14
Produced by FastQC (version 0.11.5)