FastQCFastQC Report
Sat 18 Mar 2023
SRR4895442_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895442_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635241
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAACA638500.0143.166951
GAACAAT227900.0142.837253
GGAACAA644950.0141.735172
GAACAAG200850.0141.725373
AACAATG75800.0139.449114
ACAATAG21500.0137.217225
ACAATGA21650.0136.266515
AACAAGT65050.0135.83584
GAACAAC66800.0135.724183
ACAATGT27000.0135.382695
ACAATGG24400.0135.358675
ACAAGTG20000.0134.196765
ACAAGAT21200.0133.049565
ACAATCG6600.0133.00845
CAAGATA6550.0132.925176
CAATATG7250.0132.000896
ACAATTG25650.0131.848045
ACAAGGT23150.0131.1675
CAATGAT7250.0131.00846
ACAAGTA20500.0130.572665
Produced by FastQC (version 0.11.5)