FastQCFastQC Report
Sat 18 Mar 2023
SRR4895442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635241
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34420.5418416002745414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG12890.2029151141062998No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC11560.18197817836065366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9630.15159600844403934No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG7790.12263062365307024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA7510.11822284770661844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT142600.0138.402761
GGTATCA142900.0137.852398
GTGGTAT143550.0137.37866
TGGTATC144200.0136.859187
GTATCAA144550.0136.328649
AGCAGTG144850.0136.203192
CAGTGGT145350.0135.974434
AGTGGTA145350.0135.62785
GCAGTGG147950.0133.446693
GTATCCA1102.5416276E-432.7151649
TCAACGC142800.028.22484610-14
ATCAACG142600.028.1231110-14
AGAGTAC143600.028.07762715-19
TACATGG143450.027.99141920-24
GTACATG143650.027.97249220-24
CAGAGTA144450.027.87254715-19
CAACGCA144950.027.83598510-14
AGTGGTC1306.762507E-427.682065
AGTACAT144800.027.68073520-24
GCAGAGT145800.027.6539615-19
Produced by FastQC (version 0.11.5)