FastQCFastQC Report
Sat 18 Mar 2023
SRR4895441_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895441_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691443
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG693550.0143.441931
GAAGAGA698350.0142.456012
AAGAGAT235100.0141.286853
AAGAGAG219450.0138.380313
AAGAGAC81450.0136.821663
AAGAGAA184600.0134.105773
AGAGACT20650.0129.255314
AGAGATG80350.0128.576934
AGAGATA75600.0128.471424
AGAGATT77200.0127.7658164
AGAGAAT65450.0127.0699164
GAGATAG24400.0125.9019855
AGAGAGT70000.0125.696424
AGAGAAG65800.0125.4099664
GAGAAGT21250.0124.928635
GAGATAT26550.0124.106775
GAGATTG27650.0123.852955
AGAGATC26750.0123.716774
GAGAATA19300.0123.38585
GAGATAA21750.0122.718265
Produced by FastQC (version 0.11.5)