FastQCFastQC Report
Sat 18 Mar 2023
SRR4895441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691443
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38560.5576743129947082No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCTCGGGTCGCTGCAGAC13360.1932191084442246No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC9300.13450132548886892No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC7760.11222906298856161No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGAATCCAGTGTTCTGTC7740.11193981282621994No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7480.10817956071577846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATGCAAATGTTTTGAAA6940.10036980633255381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT236850.0140.771791
GTGGTAT238600.0139.789056
GGTATCA238700.0139.54968
TGGTATC239800.0139.089517
AGTGGTA240750.0138.570545
AGCAGTG240700.0138.490222
GTATCAA240800.0138.392369
CAGTGGT242700.0137.546144
GCAGTGG245800.0135.675343
CGTGGTA850.002843653833.8637355
TACGCAC651.8189894E-1228.78501150-54
CGTACGG255.385933E-428.7850150-54
TCAACGC238900.028.43476710-14
ATCAACG239000.028.37469510-14
AGAGTAC239450.028.32738115-19
CAACGCA240350.028.31112710-14
TACATGG239900.028.23319420-24
CAGAGTA240350.028.20933215-19
GTACATG240950.028.1221120-24
AACGCAG242250.028.11284410-14
Produced by FastQC (version 0.11.5)