FastQCFastQC Report
Sat 18 Mar 2023
SRR4895440_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895440_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054834
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCAC1059050.0143.317841
CTTCACA1059100.0143.311072
TTCACAG327200.0142.751883
TCACAGT106600.0142.74794
TCACATG110300.0142.590714
TTCACAT346650.0142.400913
TCACAGG107250.0141.480274
TCACATA98000.0141.472554
TCACAGA89100.0141.311314
TCACATT109150.0140.401734
TCACAAT95550.0140.280984
TTCACAC117100.0139.655843
TCACAAG90500.0139.204794
TTCACAA283950.0138.599623
CACATAG32900.0138.209465
CACATGG38600.0137.185155
CACAGTG36800.0137.052445
TCACACG31200.0136.746644
TCACACT30650.0136.618364
TCACATC32550.0136.601064
Produced by FastQC (version 0.11.5)