FastQCFastQC Report
Sat 18 Mar 2023
SRR4895440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054834
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58920.5585713012663603No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC38100.3611942732221373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG29170.2765364028842453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG22140.20989084538420266No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC12930.1225785289438907No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA10940.10371300128740636No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT10560.1001105387198365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152000.0136.925898
AAGCAGT152550.0136.670931
GTGGTAT152800.0136.2096
GTATCAA153450.0135.632039
TGGTATC153600.0135.546437
CAGTGGT156700.0133.094544
AGCAGTG157500.0132.329832
AGTGGTA158150.0131.555735
GCAGTGG161700.0128.759083
TCAACGC151350.028.13044510-14
ATCAACG151300.028.00655710-14
AGAGTAC151950.027.93411615-19
GTACATG152700.027.79407720-24
CAGAGTA153200.027.7531715-19
TACATGG152950.027.74864620-24
CAACGCA153900.027.69240610-14
AGTACAT154600.027.38730820-24
GAGTACA152150.027.36466620-24
CGCAGAG155250.027.31253215-19
GCAGAGT156250.027.22985315-19
Produced by FastQC (version 0.11.5)