FastQCFastQC Report
Sat 18 Mar 2023
SRR4895439_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895439_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3616213
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA3620250.0143.756842
CGTCTAG1123800.0143.687333
CGTCTAA931900.0143.469993
CGTCTAT1192250.0143.441043
TCCGTCT3629800.0143.378621
CGTCTAC380250.0142.649933
GTCTAAG309050.0142.474234
GTCTATA340200.0142.331454
GTCTATG402500.0142.290944
GTCTAGG379600.0142.287584
GTCTAGA300000.0142.070894
GTCTAAT307850.0142.001114
GTCTAGT366350.0141.79654
GTCTACG106600.0141.015184
TCTAGTA109850.0139.594425
GTCTAAA270150.0139.416244
TCTATGA107450.0139.296945
GTCTATT364850.0139.223794
TCTAGAG99600.0138.788255
TCTATAG116500.0138.173485
Produced by FastQC (version 0.11.5)