FastQCFastQC Report
Sat 18 Mar 2023
SRR4895439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3616213
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182640.505058745156881No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC117640.3253126959059104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG109390.3024987742702103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT40930.11318470455141884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT878050.0139.649281
GGTATCA884750.0138.719928
GTGGTAT885150.0138.657246
TGGTATC887400.0138.386787
GTATCAA889650.0137.980169
CAGTGGT897150.0136.850744
AGCAGTG896850.0136.673752
AGTGGTA902400.0135.982795
GCAGTGG910650.0134.697483
TCAACGC883650.028.32588410-14
ATCAACG882950.028.28801710-14
AGAGTAC888800.028.0622715-19
TACATGG890700.027.9649520-24
CAGAGTA892550.027.94114115-19
GTACATG892200.027.92620-24
CAACGCA897100.027.92205810-14
AGTACAT895200.027.78255720-24
GCAGAGT898200.027.77980615-19
CGCAGAG903350.027.61187215-19
GAGTACA888250.027.54607820-24
Produced by FastQC (version 0.11.5)