FastQCFastQC Report
Sat 18 Mar 2023
SRR4895438_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895438_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183148
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGA14700.0143.805594
TCAGCAT59200.0143.805593
GCATCGA450.0143.805596
CAGCGTA100.007263583143.805577
GCACGCT400.0143.805576
CACTGAG151.2695257E-4143.805577
CATTACG100.007263583143.805577
GCATTAC202.2152562E-6143.805576
AATGACG100.007263583143.805578
CATAGGT400.0143.805577
CATAGCT100.007263583143.805577
CAATCGA151.2695257E-4143.805577
GCATGCG306.85759E-10143.805576
AGCAGTC1550.0143.805575
CACTTCG100.007263583143.805577
CATCTGA253.8864528E-8143.805567
CATCGAT253.8864528E-8143.805567
GCACTAC253.8864528E-8143.805566
TATCAGC183200.0143.727071
TCAGCAG56450.0143.67823
Produced by FastQC (version 0.11.5)