FastQCFastQC Report
Sat 18 Mar 2023
SRR4895437_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895437_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6689476
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACAT6700250.0143.663121
CCACATA6706050.0143.538862
CACATAG2123600.0143.454623
CACATAT2121600.0143.142213
CACATAA1750750.0142.345763
ACATAGT668950.0142.260244
ACATAGG739850.0142.222434
ACATATG714300.0142.16264
ACATAAG597450.0141.6074
ACATAGA569000.0141.113254
CACATAC745050.0140.978733
ACATACG215050.0140.716724
CATATAG207050.0140.524435
ACATATA609650.0140.472634
CATAGAG187250.0140.283285
CATAGTA197000.0140.133335
CATATGG247850.0140.121345
CATAGAT190100.0139.883265
ACATAGC171500.0139.699864
CATAGGT239000.0139.680575
Produced by FastQC (version 0.11.5)