FastQCFastQC Report
Sat 18 Mar 2023
SRR4895437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6689476
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA342660.5122374308540759No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG335510.5015490002505428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC240310.3592359102566479No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT116090.1735412459810006No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT111410.1665451823132335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1736550.0139.514531
GGTATCA1745300.0138.76948
GTGGTAT1748050.0138.571676
TGGTATC1750300.0138.438777
GTATCAA1755750.0137.918879
CAGTGGT1767500.0137.156724
AGCAGTG1769600.0136.892612
AGTGGTA1778850.0136.192585
GCAGTGG1802000.0134.39933
TCAACGC1740250.028.41090610-14
ATCAACG1740850.028.33414310-14
AGAGTAC1757450.028.0765315-19
TACATGG1755450.028.04481320-24
CAACGCA1765850.028.02429610-14
CAGAGTA1762600.028.00674415-19
GTACATG1762400.027.96117820-24
GCAGAGT1775100.027.79654715-19
AGTACAT1771750.027.78030420-24
CGCAGAG1775950.027.76136215-19
GAGTACA1754350.027.54374320-24
Produced by FastQC (version 0.11.5)