FastQCFastQC Report
Sat 18 Mar 2023
SRR4895436_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895436_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294265
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG1297050.0143.581621
AGGATGA1298200.0143.454442
GGATGAT456350.0142.788673
GGATGAG353250.0142.527663
GGATGAA355550.0142.354393
GGATGAC141550.0142.062473
GATGATA141050.0137.669344
GATGACC41050.0137.232214
GATGAAG109750.0137.205694
GATGACT36150.0136.926514
GATGACG37750.0136.840584
GATGAAT127250.0136.711794
GATGATG142900.0136.541564
GATGAGA102250.0135.730154
GATGACA32150.0135.61234
GATGATC46550.0135.237084
GATGATT147450.0135.2074
GATGAGG116100.0135.092654
GATGAGT118350.0134.83444
GATGAGC36100.0133.728134
Produced by FastQC (version 0.11.5)