FastQCFastQC Report
Sat 18 Mar 2023
SRR4895436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294265
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64980.5020610153253005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGTGGTGGCTCA21660.16735367177510016No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC14700.1135779766894724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC14070.10871034911706644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199800.0138.244138
GTGGTAT201850.0136.947056
GTATCAA202300.0136.53579
TGGTATC202700.0136.33737
AAGCAGT203450.0136.15071
AGTGGTA204550.0135.104235
AGCAGTG207400.0133.522932
CAGTGGT207400.0133.455874
GCAGTGG216350.0127.89953
TCAACGC199250.028.24526210-14
ATCAACG199700.028.13116310-14
AGAGTAC200600.028.01929915-19
CAGAGTA201200.027.92858915-19
CAACGCA202400.027.86256410-14
GTACATG201800.027.77331520-24
TACATGG202750.027.65738120-24
AGTACAT202950.027.62303420-24
CGCAGAG204800.027.45874215-19
GAGTACA200850.027.43876520-24
AACGCAG205800.027.40924510-14
Produced by FastQC (version 0.11.5)