FastQCFastQC Report
Sat 18 Mar 2023
SRR4895435_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895435_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805377
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCTA1811950.0143.382772
GTTCTAG537550.0143.157363
GTTCTAT623300.0142.452413
GTTCTAA474850.0142.421973
GTGTTCT1828900.0142.053911
GTTCTAC186550.0141.669863
TTCTAGA144700.0141.072564
TTCTATG210000.0139.932374
TTCTAGG172250.0138.76894
TTCTAGT188200.0138.707344
TCTAGTA53500.0138.661745
TTCTACG51150.0138.561434
TCTATGA54750.0138.255815
TCTAGAG45100.0138.163445
TCTATGG69400.0137.789865
TTCTATA179950.0137.78924
TCTAGGT58150.0137.596315
TCTATAG58700.0137.532875
TCTAGAT52550.0137.471535
CTAGTGA13950.0137.20166
Produced by FastQC (version 0.11.5)