FastQCFastQC Report
Sat 18 Mar 2023
SRR4895435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805377
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90640.5020558033031328No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC65010.360090994844844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG58580.3244751650209347No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT31210.1728724803739053No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT22230.12313217682511741No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC20610.11415898175284164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA390900.0137.702718
GTGGTAT390850.0137.701926
AAGCAGT391800.0137.597461
TGGTATC393350.0136.936527
GTATCAA394250.0136.696959
CAGTGGT398750.0135.027944
AGCAGTG400350.0134.5332
AGTGGTA401850.0133.878815
GCAGTGG410650.0131.123573
TCAACGC391250.028.16710710-14
ATCAACG391100.028.10429810-14
AGAGTAC392650.027.94569815-19
CAGAGTA394100.027.83922615-19
CAACGCA396400.027.7975310-14
TACATGG394400.027.78282720-24
GTACATG395750.027.70988320-24
GCAGAGT397400.027.61167315-19
AGTACAT397900.027.52035320-24
CGCAGAG400000.027.44299315-19
GAGTACA392800.027.3572120-24
Produced by FastQC (version 0.11.5)