FastQCFastQC Report
Sat 18 Mar 2023
SRR4895434_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895434_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946700
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCA1953450.0143.498352
CTGTCAA578300.0143.311173
CTGTCAT725750.0143.291553
CTGTCAG649950.0143.26483
GTCTGTC1956800.0143.252691
TGTCATA214300.0141.912924
TGTCATG253350.0141.836124
TGTCAAT210550.0141.773224
TGTCAGA186350.0141.60054
TGTCAGG229400.0141.422324
GTCAGGA64050.0141.41055
GTCATAG73700.0141.260595
TGTCAAG200250.0141.11964
GTCATGA66600.0141.077095
GTCAGAT69450.0140.885855
TGTCAGT242100.0140.69494
GTCAGAG62750.0139.750635
GTCAATA61850.0139.456025
GTCATGG92150.0139.386175
GTCATTA77800.0139.36885
Produced by FastQC (version 0.11.5)