FastQCFastQC Report
Sat 18 Mar 2023
SRR4895434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946700
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA96750.4969949144706426No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG76840.39471926850567624No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC50800.2609544357117173No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT45430.23336929162171882No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT29090.1494323727333436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT23220.11927877947295422No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA22510.11563158165099913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA20570.10566599886988237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT19950.10248112189859764No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC19820.10181332511429597No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT19750.10145374223044126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT429250.0139.273151
GTGGTAT431050.0138.300286
GGTATCA431350.0138.220768
TGGTATC432700.0137.756267
GTATCAA435850.0137.32239
AGTGGTA438550.0136.033585
AGCAGTG439900.0135.410312
CAGTGGT440400.0135.38044
GCAGTGG446150.0133.497223
ATCAACG432650.028.25336310-14
TCAACGC433900.028.21511310-14
CAACGCA435600.028.1876410-14
AGAGTAC433800.028.09879715-19
TACATGG430800.028.0808420-24
CAGAGTA434100.028.07606115-19
GTACATG433350.027.9388620-24
CGCAGAG438850.027.79186215-19
AGTACAT438300.027.73831720-24
GCAGAGT439150.027.68434315-19
AACGCAG444800.027.5819610-14
Produced by FastQC (version 0.11.5)