FastQCFastQC Report
Sat 18 Mar 2023
SRR4895433_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895433_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68484
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC1490.21756906722738156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGA100.0072252452143.994899
GAGTGAA253.8298822E-8143.994897
GATAAGG202.1908563E-6143.994897
AGATAAG900.0143.994896
TATGGTA100.0072252452143.994899
TGTATAG100.0072252452143.994899
AATATAG100.0072252452143.994898
GATGGTA253.8298822E-8143.994897
GATATAG100.0072252452143.994897
ATAAGTG202.1908563E-6143.994898
ATGGTAG151.2594633E-4143.994898
TAGATAA2150.0143.994895
ATAGTGA151.2594633E-4143.994898
AGGATGA151.2594633E-4143.994898
GAATTAT151.2594633E-4143.994897
GAGATTA202.1908563E-6143.994897
GATAGTA151.2594633E-4143.994897
GAAGGAA100.0072252452143.994897
CGTAGAG23400.0143.994873
AGAGATA700.0143.994876
Produced by FastQC (version 0.11.5)